Further morphological assessment after DHN treatment revealed characteristic pyroptotic features, including cell swelling and the formation of large bubbles from the plasma membrane, as indicated by red arrowheads in Figure 1B. This DHN-induced characteristic pyroptotic morphology was closely associated with LDH release and cleavage of the pyroptotic executor GSDME in melanoma A375 cells and other cancer cell lines (Figure 1B and Supplemental Figure 1A; supplemental material available online with this article; https://doi.org/10.1172/JCI188872DS1). Here, GSDME is linked to cancer.