To explore this possibility, we used TOsk-Gal4 (a combination of osk-Gal4 and αTub67C-Gal4) to drive sakura RNAi knockdown in germline cells after the germline cyst stage (from germarium region 2b onward, Figure 1A), sparing GSCs and germline cysts (ElMaghraby et al., 2022). This evidence concerns the gene LGALS4 and cyst.