This fully automated method demonstrated strong agreement with IIFT-BIOCHIP and excellent diagnostic performance in distinguishing between pemphigus and BP patients, achieving AUC values of 0.92 (95%CI: 0.92~0.98) (anti-Dsg1 or anti-Dsg3 antibody) and 0.84 (95%CI: 0.77~0.91) (anti-BP180 or anti-BP230 antibody). The gene discussed is COL17A1; the disease is pemphigus.