To test if activation of α7-nAchRs activity reduces synapses engulfing CD68+ microglia/macrophages and GFAP+ reactive astrocytes, we analyzed the number of CD68+ activated microglia/macrophages, GFAP+ reactive astrocytes, CD68+/SYN+ and GFAP+/SYN+ cells in the hippocampi of PHA treated young mice subjected to tibia fracture 6 hours before ischemic stroke at 8 weeks after the injuries. This evidence concerns the gene GFAP and ischemic stroke.