To confirm the role of endothelial 12/15-LOX in the development of atherosclerosis in disturbed flow areas, 12/15-LOX expression in endothelium was specifically knocked-down by intravenous injection of AAV-mediated delivery of ALOX15 -shRNA under the EC-specific Cdh5 promoter (Fig. 6C and Supplementary Fig. 8A). The gene discussed is LOX; the disease is atherosclerosis.