To further confirm the role of endothelial 12/15-LOX, we established EC-specific 12/15-LOX knockdown mice through AAV-shRNA controlled by an EC-specific Cdh5 promoter, which also exhibited a significant alleviation of disturbed flow-induced atherosclerosis after EC-specific 12/15-LOX knockdown. This evidence concerns the gene CDH5 and atherosclerosis.