To determine the role of Il1rl1 in leukemia maintenance, we established primary leukemia in immunocompetent mice by injecting 103 LSCs from Il1rl1f/f Mx1Cre or Il1rl1f/f MLL-AF9GFP and waited 4 days for the leukemia to be engrafted. The gene discussed is IL1RL1; the disease is leukemia.