NFKB1 and obesity due to melanocortin 4 receptor deficiency: In conclusion, this study revealed that G-SDF and SDFfbs can promote weight loss, reduce lipid accumulation, strengthen the intestinal barrier, inhibit the LPS/TLR4/MyD88/NF-κB signaling pathway through the modulation of the intestinal flora, and resist the double damage caused by obesity and antibiotics.