Similarly, scRNA-seq and spatial transcriptomics have been used to show that colorectal cancer metastases to the liver and ovary involve distinct tumor subclusters with unique molecular interactions tailored to the respective organ microenvironments; tumor cells exhibiting DLL4–NOTCH signaling were associated with ovarian colonization, whereas tumor cells with similar expression patterns to cholangiocytes were associated with liver metastasis [89]. This evidence concerns the gene DLL4 and neoplasm.