ACTL6A and soft tissue sarcoma: RMS is an aggressive pediatric soft tissue sarcoma characterized by rapid growth and poor differentiation.100,101 Studies have shown that ACTL6A is significantly overexpressed in RMS tumors compared to normal muscle tissue, highlighting its potential role in RMS development.33 ACTL6A has been identified as a direct target of miR-206, and its persistent expression in myoblasts disrupts normal muscle differentiation.102 Knocking out ACTL6A in RMS cells leads to increased expression of muscle markers, decreased cell proliferation, and reduced anchorage-independent growth.