The analysis revealed that changes in CD3+CD4-CD8- lymphocytes were influenced by pathology type (p = 0.026), with chondrosarcoma patients showing more pronounced increases (46.5% ± 16.7%) compared to chordoma (10.0% ± 16.9%) and other tumor types (7.63% ± 21.2%). This evidence concerns the gene CD4 and chordoma.