To investigate if the EBI2/oxysterol pathway is involved in the regulation of the BBB function in the context of MS, we built spheroids with the HASTRs, HBPCs and HBMECs, and treated them in vitro with CSF and various EBI2/oxysterol pathway modulating compounds (Fig. 3A and B). This evidence concerns the gene GPR183 and myeloid sarcoma.