To further discern the roles of CK2α and FUNDC1 in the etiology of MASLD, CK2α‐/‐, FUNDC1‐/‐, CK2α‐/– FUNDC1‐/‐ double knockout and WT mice were offered either a low‐fat diet (LFD) or HFD diet for 20 weeks. This evidence concerns the gene FUNDC1 and metabolic dysfunction-associated steatotic liver disease.