GPT and fatty liver disease: HFD intake also led to significant liver steatosis (visualized by Oil Red O staining, Figure 4A), elevated levels of AST, ALT, triglycerides, and cholesterol, as well as increased hepatic lipid droplet formation (both in number and size), plasma insulin and the homeostasis model assessment of insulin resistance (HOMA‐IR) index, while plasma albumin levels remain unchanged (Figure 4B–J).