To decipher the possible role of mitophagy and ferroptosis in HFD‐induced changes in hepatic steatosis, we transfected both HepG2 cells and primary murine hepatocytes using siRNA targeting CK2α and FUNDC1 (scramble RNA serving as the control) before subjecting the cells to a 72‐hr palmitic acid (100 μM) challenge, with or without mitophagy suppressor liensinine, ferroptosis blocker liproxstatin‐1 (LIP1), or ferroptosis activator erastin. This evidence concerns the gene FUNDC1 and steatosis.