MECP2 and Anxiety: The effect of environmental enrichment on anxiety in mice with Mecp2 mutations emphasises that standardised animal husbandry practices are important in securing the reproducibility of results (Katz et al., 2012; Kondo et al., 2016; Kerr et al., 2010) and the maternal separation study indicates that maternal temperament may affect anxiety (Abellán-Álvaro et al., 2021).