TLR4 and necrotizing enterocolitis: Using the “in vitro neutrophil organoid system” to model neutrophil epithelial cell interactions, this study suggests that even though neutrophils did not seem to trigger higher apoptosis rates when added to unstressed organoids (without LPS), irrespective of whether organoids were derived from CO or NEC affected tissue, an increase in intestinal organoid TLR-4 expression was observed when neutrophils were added to stressed organoids.