TP53BP1 and synucleinopathy: Quantitation of nuclear 53BP1 levels revealed significantly more (p<0.0001, Unpaired-T test, t=5.215) 53BP1 puncta in mouse synucleinopathy tissue, with an average of 66.89 puncta per cell (95% CI 54.89–78.89) while control cortical cells averaged 29.64 (95% CI 22.10–37.18) 53BP1 puncta per cell (Fig. 4A, Right).