In the present study, the α‐SMA expression level in the CCl4, colchicine, L‐Fx, and H‐Fx groups was 169.92%, 119.19%, 82.41%, and 96.83% of the control group (set at 100%), respectively (Figure 4D), suggesting that colchicine and Fx are effective in treating CCl4‐induced liver fibrosis in mice. The gene discussed is ACTA1; the disease is Hepatic fibrosis.