Based on the expression of CHRNA7 in CD45+ cells of spleen, peripheral blood and tumors, and the role of CHRNA7 in transcriptional signaling,9 we isolated tumor-associated CD45+ cells by magnetic beads from the primary E0771 tumors of CHRNA7KO and CHRNA7WT mice and performed a NanoString immune cell–related gene panel analysis (Fig. 2E). Here, CHRNA7 is linked to neoplasm.