HSPG2 and hepatocellular carcinoma: To evaluate the therapeutic potential of targeting GSDME in HCC, we constructed the mice subcutaneous xenograft model using HCC cell lines with stable GSDME overexpression (PLC/PRF/5 OE-GSDME) and their corresponding control (PLC/PRF/5 control), as well as HCC cell lines with stable GSDME downregulation (SK-Hep-1 sh-GSDME) and their control (SK-Hep-1 control).