A2M-AS1 and infection: In order to investigate the functional role of A2M‐AS1 in PC, we stably overexpressed A2M‐AS1 in PANC‐1 cells with low levels via lentiviral infection and stably transduced BxPC‐3 cells with lentiviral shRNA constructs targeting A2M‐AS1, as confirmed by qRT‐PCR analysis (Figure 1E,F).