METTL3 and hepatocellular carcinoma: To determine the precise effect of METTL3 O-GlcNAcylation on HCC development, we knocked down endogenous METTL3 in HCC cell lines using an shRNA targeting its 3′-UTR and then reintroduced either WT or the 3A mutant of METTL3 using adenoviral vectors (Fig. 3A; Fig. S3A).