GCLC and renal cell carcinoma: In addition, results of wound healing assays in ACHN and A498 cells showed that overexpression of GCLC significantly restored the migratory capability of RCC cancer cells against CENPT shRNA (fig S8L–O), and transwell assay also showed that overexpression of GCLC markedly rescued the inhibitory effects of CENPT shRNA on RCC cancer cell migration and invasion (Fig. 4L–O).