Subsequently, overexpression lentiviruses targeting CENPT were stably transfected into 786-O and Caki-1 cell lines (the overexpression efficiency of the transfected RCC cells was shown in fig S1C–E), while CENPT shRNA lentiviruses were stably transfected into A498 and ACHN cells (the target sequences of CENPT shRNA were shown in Table S4, and the knockdown efficiency of the transfected RCC cells was shown in fig S1F–I). This evidence concerns the gene CENPT and renal cell carcinoma.