After 8 months of normal diet feeding, HE staining results showed that the liver tissue structure was basically normal in Men1ΔM/ΔM and WT groups, but the IHC results revealed that SR, α-SMA staining, and F4/80+ MAC infiltration were increased in the Men1ΔM/ΔM group (Fig. S2J), suggesting that Men1ΔM/ΔM results in a propensity for spontaneous liver fibrosis. This evidence concerns the gene ACTA1 and Hepatic fibrosis.