HCC cells HLE, HLF, and JHH6 were classified to subtype 1/transforming growth factor beta-Wingless related integration site (S1/TGFβ-Wnt) activated subtype, while Huh7 and HepG2 were classified as subtype 2 (S2/progenitor subtype) [6,13]. The gene discussed is TGFB1; the disease is hepatocellular carcinoma.