SOD2 and Zinc deficiency: Lai et al. used an in vitro experimental model adding N,N,N′,N′-Tetrakis (2-pyridylmethyl) ethylenediamine (TPEN) to show that zinc deficiency impaired mitochondrial function, leading to increased ROS and acetylated superoxide dismutase 2 (SOD2), which resulted in early apoptosis [82].