The two studies measuring NT-proBNP during the first trimester found an inverse relationship; three studies measuring NT-proBNP during the second trimester did not find a significant relationship; one study, measuring NT-proBNP during the third trimester found elevated levels were associated with a higher risk of developing preeclampsia within 4 weeks but with a decreased risk when the timeframe extended beyond 4 weeks. The gene discussed is NPPB; the disease is preeclampsia.