The state of five pathways—STAT3; Extracellular Signal-Regulated Kinase (ERK)/mitogen-activated protein kinase (MAPK); PI3K/Akt; Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB); and Notch- in U266 and RPMI8226 cells, cultured in 3D or conventionally—has been investigated to evaluate whether the 3D culture shows a substantial effect on cellular signaling in MM cells [64]. Here, STAT3 is linked to Miyoshi myopathy.