Figure 6 shows both the entire tumor slice and an expanded section of 133R (Figure 6A,B,E,F) and 295R (Figure 6C,D,G,H) where the upper panels are stained with F5 and the lower panels with isotype control mAb. Figure 6A,B shows strong cytoplasmic and membranous staining of the neoplastic cells in the 133R sample and virtually no staining of 295R (Figure 6C,D). Figure 6E–H show very little staining with the isotype control. These results were consistent across multiple tumors evaluated showing statistically significant differences in MUC4 percent positive and H-score (Supplemental Figure S5). Here, MUC4 is linked to neoplasm.