To investigate the mechanism of quinazolinedione derivatives in MCF-7 breast cancer cells through the early apoptosis signaling pathway, the phosphorylated forms of JNK, Akt, Bad, Bcl-2, p53, active caspase-8, and active caspase-9 were captured using magnetic beads and detected by the Luminex® system. Here, AKT1 is linked to breast carcinoma.