Using a model of liver fibrosis and focusing on the TGF-β/SMAD2/3 signaling pathway, we observed that the exposure of TGF-β1-activated HSC to 3-HBI downregulated the pro-inflammatory cytokines ACTA2, TIMP1, COL1A1, COL4A1, SMAD2, SMAD3, SMAD4, MMP2, and MMP9. Here, SMAD2 is linked to Hepatic fibrosis.