To complement immunohistochemical and functional in vitro data, quantitative gene expression analysis using Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) could serve as a valuable method to validate the observed protein-level differences—particularly for YAP, TAZ, and TEAD4—on the mRNA level, and to explore their correlation with tumor subtype or stage. The gene discussed is YAP1; the disease is neoplasm.