As a result, this research utilized 11 serotonin derivatives to assess the effect of NO on LPS-stimulated RAW 264.7 cells and its role in regulating crucial cytokines linked to ulcerative colitis, specifically IL-6, TNF-α, IL-1β, and IL-10, through Griess and RT-qPCR assays. The gene discussed is IL1B; the disease is ulcerative colitis.