GPC3 and hepatocellular carcinoma: Therefore, isolating GPC3-positive CTCs using immunomagnetic enrichment, followed by fluorescent active cell sorting and anti-cytokeratin (CK) (anti-CK7, anti-CK9, anti-CK20, and anti-CK8) staining, has been considered to be a significant approach for patients with HCC, an epithelial marker, and antibodies.