Overexpression of APPLE in HepG2 and Huh7 cells significantly enhanced cell proliferation (Fig. 3A-C), migration (Fig. 3D), invasion (Fig. 3E), tumor biomarker levels (AFP and PCNA, Fig. 3F&G), and tumorigenic ability in vitro and in vivo (Fig. 3H) compared to control cells. Here, AFP is linked to neoplasm.