ASH1L-AS1 and hepatocellular carcinoma: In order to investigate the role of APPLE in HCC, we established stable HepG2 and Huh7 cell lines overexpressing APPLE (5‘-UTR-ORF-Flag) or a start codon-mutated version (5’-UTR-ORFmut-Flag), as well as ASH1L-AS1 knockdown cell lines.