ZC3HAV1 and colorectal carcinoma: Having demonstrated that the loss of PARP13 is associated with increased CRC cell sensitivity to multifractionated irradiation in a microenvironment dependent manner, we went on to the level of whole genome transcriptome to compare the relative abundance of transcripts in HT29 PARP13(−/−) cells to wild type cells cultured in 2D and lr-ECM 3D model systems (Fig. 3A).