Commencing with the LLPS mechanism, this study identified five LLPS-related genes (ANXA10, MYC, TIMM8A, WASF3, and WDR3) with prognostic potentials through bioinformatics approaches, and the corresponding risk model constructed was capable of predicting the survival likelihood of patients with osteosarcoma. This evidence concerns the gene WASF3 and osteosarcoma.