We stained two sequential tumor sections – one using IHC for TMEM doorways (Iba1, Endomucin, Mena) (Fig. 1B) and the second using IF for CSF-1, dextran, and endothelial cells (endomucin) (Fig. 1C) and aligned the images of stained sections to the single cell level. The gene discussed is CSF1; the disease is neoplasm.