To determine if the enhancement of VEGF-A secreted by the macrophages in response to co-culture with tumor cells could also be due to increased VEGF-A mRNA production, we co-cultured MDA-MB-231 tumor cells with BAC1.2F5 macrophages and determined the relative levels of VEGF-A mRNA by qPCR. Here, VEGFA is linked to neoplasm.