PTPRC and neoplasm: In order to understand the TIM3+ tumor cell-mediated immune-evasive effects during micrometastasis (in 4T07 Ctrl and Tim3-KD conditions), we isolated CD45+ immune cells as described56 and we performed single-cell RNA-seq from micro- and macro-metastatic liver and adjacent tissue (AT) (Figure 5A and S5A–S5C).