During TIL culture, enzymatic digestion of tumor tissue may have reduced suppression mediated by direct cell–cell contact, and the use of high-dose IL-2 (6000 U/mL) likely helped to overcome IL-2 competition by Tregs [40], allowing even functionally impaired T cells to expand and regain IFN-γ production capacity. The gene discussed is IFNG; the disease is neoplasm.