To identify expression patterns and pathways implicated in ALS across different mutations, we performed whole-transcriptome sequencing (RNA-seq) analysis on technical duplicates from iPSCs and neurospheres, from the four patients carrying a pathogenetic mutation in one ALS gene, namely KIF5A (ALS_001), TARDBP (ALS_002 and ALS_004), and C9orf72 (ALS_003), and two healthy gender-matched controls (H00_1 and H00_3), for a total of 24 samples. Here, TARDBP is linked to amyotrophic lateral sclerosis.