MAP2K4 and hepatocellular carcinoma: BI-D1870 has a relatively high cytotoxicity, whichhas been linked to its off-target activity., Therefore, BI-D1870 and MKK4 inhibitors 39 and 45 were tested on their cytotoxicity in an XTTcell viability assay with NrasG12 V;Cdkn2aARF–/– HCC cells and NCI–H2030 NSCLC cells, which were exposed tothe compounds in concentrations from 13.7 nM–90 μM (45.7nM–30 μM for NCI–H2030) for 72 h.