TNF and neoplasm: To further characterize the ability of these newly derived cells to spontaneously secrete chemokines and cytokines that can shape up the immune tumor microenvironment (TME), we interrogated the cell culture supernatants of 25 cell lines, for seven different secreted markers (IP-10/CXCL10, RANTES/CCL5, MCP-1/CCL2, eotaxin/CCL11, MIP2/CXCL2, TNFα/TNF and VEGF), using Meso Scale Discovery (MSD) multiplex technology (Fig. 6B; Fig. S8A).