Finally, by analyzing the correlation between microbiota and inflammatory markers (Figure 8C), we found that 21 bacterial genera such as g__unclassified_o__Oscillospirales and g__Denitrobacterium showed a significant negative correlation with TNF-α, IL-1β and MPO, which suggested that these bacterial genera may play a positive role in alleviating mastitis. Here, TNF is linked to mastitis.