Excessive apoptosis and migration of lens epithelial cells contribute to the development of cataracts.30, 31, 32 Therefore, the TUNEL assay revealed a decrease in positively stained cells within the lens sections of the AAV-NR2F1+ASC mice compared with those of the AAV-NC + ASC mice (Fig. 5A). The gene discussed is NR2F1; the disease is cataract.