The LSD genes associated with NIHF diagnosed by ES were as follows: GUSB (14/27, 52%), SLC17A5 (3/27,11%), CTSA (2/27, 7%), GLB1 (2/27, 7%), NPC1 (2/27, 7%), ARSB (1/27, 4%), GBA (1/27, 4%), NEU1 (1/27, 4%), and SUMF1 (1/27, 4%). This evidence concerns the gene CTSA and Non-immune hydrops fetalis.