To further explore the selective functional enrichment of the top methylated transcripts used in the network mapping, we queried the KEGG pathway database and filtered for pathways (cAMP signaling, dopaminergic signaling, axon guidance functions, cortisol synthesis, GABAergic signaling, phosphatidylinositol signaling, TNF signaling, serotonergic signaling, cholinergic synapse signaling, glutamatergic synapse, AMPK signaling, PI3K-AKT signaling, and MAPK signaling) known to be dysregulated in MDD. This evidence concerns the gene PIK3CA and major depressive disorder.