To further investigate whether FN1 regulates colon cancer migration and metastasis in vitro through RAP1B, we introduced RAP1B shRNAs in FN1‐overexpression HCT116 cells, and transfected FN1‐knockdown LOVO cells with RAP1B overexpression plasmid. This evidence concerns the gene FN1 and malignant colon neoplasm.