To examine the nuclear location of NF-κB following low MOI HCMV infection, we infected MRC-5 cells with mCherry-tagged wild-type (TB40/E-mCh), UL88-STOP (UL88-STOP-mCh), or UL88-Rev (UL88-Rev-mCh) HCMV at an MOI of 0.05. Here, NFKB1 is linked to cytomegalovirus infection.