GPX4 and brain infarction: 2019). Western blot analysis was used to quantify the expression levels of the antioxidant‐associated proteins SLC7A11 and GPX4, while the content of GSH and the lipid peroxidation product MDA were assessed to determine the extent of lipid peroxidation. As shown in Figure 7, at 6 h after rtPA thrombolysis in cerebral infarction, the levels of the negative regulators GSH, GPX4, and SLC7A11 were reduced, and the level of the lipid peroxidation product MDA was increased.