At the chronic stage of infection (42 dpi), subset A increased significantly, with a corresponding decrease in subset C. In addition to identifying previously described subsets primarily characterized by lack of CD63 expression (A‐E), Toxoplasma infection also induced small novel populations of astrocytes that upregulated CD63 and expressed CD51, CD71, or both (Figure 1; novel subsets F–H). This evidence concerns the gene TFRC and infection.