To ascertain whether ICAM-1 regulates the Src-Ezrin-Cav-1 pathway via Src to jointly regulate EMCV internalization, we initially analyzed the viral titers in both wild-type (WT) BHK-21 cell lines and ICAM-1 KO BHK-21 cell lines following EMCV infection. This evidence concerns the gene EZR and infection.